Recombinant Human CD19/Leu-12 Protein (Fc Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032206-10, PKSH032206-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD19; Leu-12

Target Symptom: B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19; CVID3

Research Area: immunology; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc

UNIProt ID: P15391

Accession: P15391

Background: CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact; it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation; the cytoplasmic tail of CD19 becomes phosphorylated; which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency; hypogammaglobulinemia; recurrent bacterial infections and an inability to mount an antibody response to antigen.

Activity: Immobilized Human FMC63 at 2μg/ml(100 μl/well) can bind Human CD19-Fc. The ED50 of Human CD19-Fc is 2. 48 ug/ml.

Sequence: Pro20-Lys291

Purity: > 80 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 57.3 kDa

Observed MW: 78 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only