Recombinant Human CD122/IL-2RB Protein (Fc Tag)(Active)

Size: 100μg

Catalogue Number: PKSH031479-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD122; IL-2RB

Target Symptom: CD122; IL15RB; P70-75

Research Area: Signal Transduction; immunology; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-hFc

Accession: NP_000869.1

Background: Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Activity: 1. Measured by its ability to bind biotinylated recombinant rat IL2 in a functional ELISA.
2. Using the Octet RED System, the affinity constant (Kd) of Human IL2RB-Fc bound to Human IL2 was 0.8nM.

Sequence: Met 1-Asp 239

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 51.6 kDa

Observed MW: 60-65 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only