Human BPAG1 (Dystonin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0651-48, ELK0651-96, ELK0651-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 230 kDa bullous pemphigoid antigen; 230/240 kDa bullous pemphigoid antigen; Bullous pemphigoid antigen 1; BPA; Bullous pemphigoid antigen; Dystonia musculorum protein; Hemidesmosomal plaque protein; DST

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BPAG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BPAG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BPAG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BPAG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q03001

Research Use Only