ATP6V0D1 Polyclonal Antibody

Catalogue Numbers: BS60264-50, BS60264-100

Sizes: 50µl, 100µl

Alternative Name: V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1; ATP6V0D1; ATP6D, VPATPD

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61421

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex. Nine subunits (A–H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain. V-ATPase D1 (ATPase, H+ transporting, lysosomal, V0 subunit D1), also known as ATP6V0D1, P39, VATX, VMA6, ATP6D or VPATPD, is the D subunit of the V0 domain. Expressed ubiquitously, V-ATPase D1 acts in concert with other V0 subunits to catalytically acidify a variety of intracellular compartments, thereby synthesizing ATP to be used for vacuolar transport.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP6V0D1 polyclonal antibody detects endogenous levels of ATP6V0D1 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP6V0D1 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human ATP6V0D1.

Conjugate: Unconjugated

Modification: Unmodified