Recombinant Human FcRn & B2M Heterodimer (C-6His-Avi) Biotinylated

Sizes: 20μg, 100μg

Catalogue Numbers: PKSH033906-20, PKSH033906-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: FcRn; B2M

Target Symptom: IgG receptor FcRn; Neonatal Fc receptor; FCRN

Research Area: Cardiovascular; Cancer

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application: BLI

Fusion Tag: C-6His-Avi

Accession: AAF72596&P61769

Background: FcRn complex consist of two subunits: IgG receptor FcRn large subunit p51（alpha chain) and Beta-2-microglobulin（Beta chain). The complexes is similar in structure to MHC class I-like heterodimer. Beta-2-microglobulin involved in the presentation of peptide antigens to the immune system. FcRn binds to the Fc region of monomeric immunoglobulins gamma, mediates the uptake of IgG from milk, Possible role in transfer of immunoglobulin G from mother to fetus. A principal component of antibody transport is the neonatal receptor for the Fc portion of immunoglobulin, a heterodimer of a MHC-1 alpha-chain homolog ( FcRn ) and beta-2-microglobulin ( B2M ).

Activity: Loaded Biotinylated Human FcRn Heterodimer-His-Avi on HIS1K Biosensor, can bind Anti-Human HER2 mAb with an affinity constant of 0.11 uM as determined in BLI assay.

Sequence: Ala24-Ser297&Ile21-Met119

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 7.8.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 31.1&13.5 kDa

Observed MW: 35&13 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only