PMS2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10345-50, MB10345-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P54278

Host: Rabbit

Reactivity: Human

Applications: WB,IP

All Applications: WB: 1/500-1/1000 IP: 1/20

Background: Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PMS2 in Jurkat, HelaLysates using PMS2 antibody.

Bioworld Molecular Weight: Calculated MW: 96 kDa; Observed MW: 96 kDa

Specificity: IgG

Alternative Name: DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2

Immunogen: A synthetic peptide of human PMS2

Conjugate: Unconjugated

Modification: Unmodified